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mouse monoclonal wisp2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal wisp2
    Mouse Monoclonal Wisp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wisp2/WISP-2+Antibody/us12465618-282-21-28
    Average 93 stars, based on 21 article reviews
    mouse monoclonal wisp2 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-Cell Transcriptomics Reveals the Difference of Aortic Atherosclerosis Response to Phytosterols and Oxidation Products of Sterols.
    Article Snippet: Scope: Phytosterols (PS) and sterol oxidation products are key dietary factors influencing atherosclerosis besides cholesterol, although the mechanisms remain elusive.. Recently, single-cell RNA sequencing (scRNA-seq) has revealed the heterogeneity of multiple cell types associated with complex pathogenesis in atherosclerosis development.. Methods and results: Here, scRNA-seq is performed to investigate the alterations in the aortic cells from ApoE−/− mice induced by diet-derived PS or two sterol oxidation products, phytosterols oxidation products (POPs), and cholesterol oxidation products (COPs).

    Article Title: Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling.
    Article Snippet: Accepted Manuscript Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling Xiao-fang Han, Xin-xiu Zhang, Ke-mei Liu, Qiu Zhang PII: S0016-6480(18)30161-8 DOI: https://doi.org/10.1016/j.ygcen.2018.05.024 Reference: YGCEN 12950 To appear in: General and Comparative Endocrinology Received Date: 17 March 2018 Revised Date: 14 May 2018 Accepted Date: 22 May 2018 Please cite this article as: Han, X-f., Zhang, X-x., Liu, K-m., Zhang, Q., Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling, General and Comparative Endocrinology (2018), doi: https://doi.org/10.1016/j.ygcen.2018.05.024 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular Adipogenesis
    Article Snippet: .. The membranes were blocked with 5% ( w/v ) nonfat dried milk at room temperature for one hour, and then incubated the target proteins with following primary antibodies against ZNF423 (sc-10486, Santa Cruz Biotechnology, Santa Cruz, CA, USA), PPARγ (sc-6284, Santa Cruz Biotechnology, Santa Cruz, CA, USA), FABP4 (sc-18661, Santa Cruz Biotechnology, Santa Cruz, CA, USA), WISP2 (sc-514070, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and β-tubulin (10094-1-AP, Proteintech, Chicago, IL, USA), with 1:1000 dilution at 4 °C overnight. .. The membranes were rinsed three times and reacted with HRP-conjugated donkey anti-goat IgG (A0181, Beyotime, Shanghai, China) or HRP-conjugated goat anti-mouse IgG (A0216, Beyotime, Shanghai, China) for 1 hour.

    other:

    Article Title: Up-regulation of T-cell factor-4 isoform-responsive target genes in hepatocellular carcinoma
    Article Snippet: Enzyme-linked immunosorbent assay Direct binding duplex enzyme linked immunosorbent assay (ELISA) was performed as previously described ( 17 ).

    Blocking Assay:

    Article Title: Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling.
    Article Snippet: Accepted Manuscript Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling Xiao-fang Han, Xin-xiu Zhang, Ke-mei Liu, Qiu Zhang PII: S0016-6480(18)30161-8 DOI: https://doi.org/10.1016/j.ygcen.2018.05.024 Reference: YGCEN 12950 To appear in: General and Comparative Endocrinology Received Date: 17 March 2018 Revised Date: 14 May 2018 Accepted Date: 22 May 2018 Please cite this article as: Han, X-f., Zhang, X-x., Liu, K-m., Zhang, Q., Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling, General and Comparative Endocrinology (2018), doi: https://doi.org/10.1016/j.ygcen.2018.05.024 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Nonspecific Binding:

    Article Title: Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling.
    Article Snippet: Accepted Manuscript Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling Xiao-fang Han, Xin-xiu Zhang, Ke-mei Liu, Qiu Zhang PII: S0016-6480(18)30161-8 DOI: https://doi.org/10.1016/j.ygcen.2018.05.024 Reference: YGCEN 12950 To appear in: General and Comparative Endocrinology Received Date: 17 March 2018 Revised Date: 14 May 2018 Accepted Date: 22 May 2018 Please cite this article as: Han, X-f., Zhang, X-x., Liu, K-m., Zhang, Q., Apelin-13 deficiency alters cortical bone geometry, organic bone matrix, and inhibits Wnt/β-catenin signaling, General and Comparative Endocrinology (2018), doi: https://doi.org/10.1016/j.ygcen.2018.05.024 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Cell-Signaling:

    Article Title: Loss of WISP2/CCN5 in Estrogen-Dependent MCF7 Human Breast Cancer Cells Promotes a Stem-Like Cell Phenotype
    Article Snippet: .. The membranes were blocked with saturating buffer for 1 h at room temperature (25°C), then probed with the following specific antibodies: CD44, actin, LEF1, FOXC1, ZEB1, Claudin-3, Claudin-4, Claudin-7, E-cadherin, IGFBP7, Oct3/4, Nanog and WISP2 were obtained from Santa Cruz Biotechnology, ALDH1A1, ALDH2 and ALDH1A3 were obtained from Abcam, Sox9 was obtained from Aviva System Biology, SNAI2, p-Smad2, p-Smad3, Smad2/3, Smad4 were obtained from Cell Signaling Technology. .. Membranes were then washed and incubated with horseradish peroxidase-conjugated secondary antibodies for 2 h. Membranes were washed extensively and developed with an enhanced chemiluminescence kit (Bio-Rad) using Chemidoc system (Bio-Rad).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Wnt Signaling Pathway Proteins in Scar, Hypertrophic Scar, and Keloid: Evidence for a Continuum?
    Article Snippet: Hypertrophic scars and keloids are fibroproliferative lesions characterized by excessive collagen deposition.. It is unclear whether these entities represent distinct disorders or share a common pathogenesis and the molecular underpinnings of these lesions are poorly understood.. Accumulating evidence suggests that the Wnt signaling pathway is a key regulator of wound healing.

    Immunohistochemical staining:

    Article Title: Wnt Signaling Pathway Proteins in Scar, Hypertrophic Scar, and Keloid: Evidence for a Continuum?
    Article Snippet: Hypertrophic scars and keloids are fibroproliferative lesions characterized by excessive collagen deposition.. It is unclear whether these entities represent distinct disorders or share a common pathogenesis and the molecular underpinnings of these lesions are poorly understood.. Accumulating evidence suggests that the Wnt signaling pathway is a key regulator of wound healing.



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    Image Search Results


    qPCR validation of differentially expressed genes. ( A ) GDF5 . ( B ) CCN5 . ( C ) SKIL . For each gene, cumulative results ( n = 5 donors) are showed, together with donors clustered by response to miRNA inhibitor treatment: responders (#162, #217, #255) and non-responders (#152, #157). * p < 0.05

    Journal: Stem Cell Research & Therapy

    Article Title: Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment

    doi: 10.1186/s13287-025-04589-8

    Figure Lengend Snippet: qPCR validation of differentially expressed genes. ( A ) GDF5 . ( B ) CCN5 . ( C ) SKIL . For each gene, cumulative results ( n = 5 donors) are showed, together with donors clustered by response to miRNA inhibitor treatment: responders (#162, #217, #255) and non-responders (#152, #157). * p < 0.05

    Article Snippet: CNN5 , Hs01031984_m1 , , .

    Techniques: Biomarker Discovery

    The expression level and diagnostic efficacy of candidate markers (EFNA1, TYMS and WISP2) in CC and OC. A , C The expression level of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in CC and OC

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: The expression level and diagnostic efficacy of candidate markers (EFNA1, TYMS and WISP2) in CC and OC. A , C The expression level of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in CC and OC

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: Expressing, Diagnostic Assay

    Expression and diagnostic validation of candidate biomarkers (EFNA1, TYMS and WISP2) in CC and OC. A , C Expression comparison of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in the CC and OC

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: Expression and diagnostic validation of candidate biomarkers (EFNA1, TYMS and WISP2) in CC and OC. A , C Expression comparison of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in the CC and OC

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: Expressing, Diagnostic Assay, Biomarker Discovery, Comparison

    Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA). Protein levels of EFNA1、TYMS and WISP2 in A , E , I cervical normal tissue, and B , F , J CC tissue. Protein levels of EFNA1、TYMS and WISP2 in C , G , K ovary normal tissue, and D , H , L OC tissue

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA). Protein levels of EFNA1、TYMS and WISP2 in A , E , I cervical normal tissue, and B , F , J CC tissue. Protein levels of EFNA1、TYMS and WISP2 in C , G , K ovary normal tissue, and D , H , L OC tissue

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: